Journal:

Article Title: LPS-induced IL-6, IL-8, VCAM-1, and ICAM-1 Expression in Human Lymphatic Endothelium

doi: 10.1369/jhc.7A7299.2007

Figure Lengend Snippet: Expression of toll-like receptor 4 (TLR4), MD-2, and CD14. (A) Immunohistochemical analysis. The cell membrane was visualized with rhodamine-conjugated Con A (Con A, in red), and the reaction products to antibodies for TLR4, MD-2, and CD14 were visualized with an AF488-conjugated second antibody (AF488, in green). Merged images indicate that the expression of TLR4, MD-2, and CD14 was immunohistochemically observed in the cytoplasm and on the cell membrane of LEC, and that the three were detected on the membrane of HEK-293 cells stably transfected with the human TLR4, MD-2, and CD14 genes (tHEK), and not in the negative control HEK-293 cells (HEK). Bar = 100 μm. (B) RT-PCR analysis. RT-PCR products for TLR4, MD-2, and CD14 mRNAs were not detected in HEK-293 but were detected in LEC and in tHEK. MW, molecular weight marker.

Article Snippet: The cells were immunostained with 1 μg/ml of rabbit antiserum to human Prox1 (AngioBio Co.; Del Mar, CA) and of monoclonal antibodies to human podoplanin (AngioBio), VCAM-1, and ICAM-1 (R and D Systems, Inc.; Minneapolis, MN), and also immunostained with 5 μg/ml of monoclonal antibodies to TLR4, MD-2, and CD14 (R and D Systems).

Techniques: Expressing, Immunohistochemical staining, Membrane, Stable Transfection, Transfection, Negative Control, Reverse Transcription Polymerase Chain Reaction, Molecular Weight, Marker

Journal:

Article Title: LPS-induced IL-6, IL-8, VCAM-1, and ICAM-1 Expression in Human Lymphatic Endothelium

doi: 10.1369/jhc.7A7299.2007

Figure Lengend Snippet: Expression of genes for lymphatic endothelial markers, and TLR-associated and leukocyte adhesion molecules in human neonatal dermal lymphatic microvascular endothelial cells with LPS treatments

Article Snippet: The cells were immunostained with 1 μg/ml of rabbit antiserum to human Prox1 (AngioBio Co.; Del Mar, CA) and of monoclonal antibodies to human podoplanin (AngioBio), VCAM-1, and ICAM-1 (R and D Systems, Inc.; Minneapolis, MN), and also immunostained with 5 μg/ml of monoclonal antibodies to TLR4, MD-2, and CD14 (R and D Systems).

Techniques: Expressing, Control, Marker, Activation Assay

Journal: Frontiers in Physiology

Article Title: Using Imaging Mass Cytometry to Define Cell Identities and Interactions in Human Tissues

doi: 10.3389/fphys.2021.817181

Figure Lengend Snippet: List of antibodies used on human kidney tissue.

Article Snippet: CD14 , Monocytes/Macrophages , Rabbit , EPR3653 , 144Nd , 1:50 , Fluidigm , .

Techniques: Membrane